Fabrication and optimisation of a fused filament 3D-printed microfluidic platform

Tothill, A M and Partridge, M and James, S W and Tatam, R P (2017) Fabrication and optimisation of a fused filament 3D-printed microfluidic platform. Journal of Micromechanics and Microengineering, 27 (3). 035018. ISSN 0960-1317

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Abstract

A 3D-printed microfluidic device was designed and manufactured using a low cost ($2000) consumer grade fusion deposition modelling (FDM) 3D printer. FDM printers are not typically used, or are capable, of producing the fine detailed structures required for microfluidic fabrication. However, in this work, the optical transparency of the device was improved through manufacture optimisation to such a point that optical colorimetric assays can be performed in a 50 µl device. A colorimetric enzymatic cascade assay was optimised using glucose oxidase and horseradish peroxidase for the oxidative coupling of aminoantipyrine and chromotropic acid to produce a blue quinoneimine dye with a broad absorbance peaking at 590 nm for the quantification of glucose in solution. For comparison the assay was run in standard 96 well plates with a commercial plate reader. The results show the accurate and reproducible quantification of 0–10 mM glucose solution using a 3D-printed microfluidic optical device with performance comparable to that of a plate reader assay.

Item Type: Article
Subjects: Afro Asian Library > Multidisciplinary
Depositing User: Unnamed user with email support@afroasianlibrary.com
Date Deposited: 09 Jun 2023 06:23
Last Modified: 19 Jun 2024 12:26
URI: http://classical.academiceprints.com/id/eprint/1050

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